Details. Which Muay Thai camp, how long was your 500k sitting in the bank, is it a normal personal bank account in your name, how many months bank statement did you provide, were you asked to prove location and how did you prove it, was there any further documents requested? How old are you?
Answer each and every one of those questions in detail.
Are you asking about before the application is submitted or after the visa is approved?
Regardless it's each individual embassies policy how long the money has to be seasoned before the application, but after the Visa has been granted there is no actual rule about how long it has to stay in the account.
Number of DTV applications are rejected for various reasons and there's nothing stopping you from resubmitting your application with sufficient approvable info. That said, using the proper school with the proper documentation and the likeliness of rejection is extremely low.
If you have your own business then choose the workcation category. Draw up a contract, and employment certificate hiring yourself and authorizing yourself to work remotely in Thailand. Likely need to submit at least 3 months of personal bank statements where you can highlight your income maintaining balance of the 500K funding requirement.
Once issued the visa has a use before/validity date. You have until that date to enter the country, and then you will be stamped in for however long the visa allows for. For most visas you have 90 days from date of issue to use/enter the country.