Can my spouse apply for a Non-O visa in Penang after I obtain my Non-B visa?

You can also visit the Immigration Office and apply for the Extension of Stay for both of you, will be done in one day.

You're NOT going to "renew anything in Penang" you'd be going to the thai consulate to BUY completely NEW visas. Keep in mind they now only issue year long multi-entry Non-B visas to someone listed as the director of the company on the work permit.

There shouldn't be a problem for him to go, get his Non-B from them, then a week later you show up and get your Non-O based on being the spouse of someone on a Non-B that works here. You'd have to have ALL the documentation from him that you used last year in order (seeing as he's NOT there to provide paperwork if you need something) but it should work

You can't legally work at ALL (as in you can't even get a work permit to be allowed to work) on a Non-O based on being the "trailing spouse" of someone that has a valid Non-B and a work permit so the point is actually moot. :/

I understand, but to run the business while your husband is away you need your own Work Permit and Non Immigrant B visa.

Meaning of going seperately is that you can keep the business open?