Can I use a business account for the DTV visa in Thailand instead of a personal account?

It is you as a person, not your compagny, that applies for DTV, so it is your personal account in your name, you have to show containing 500.000 Baht.

The money need to be immediately accessible, if for instance you need to pay for hospital, before you can get treatment.

And have to have been in the account up to 6 months before applying.

When you need ask that, you will benefit by reading a lot more posts in the group before you apply, because you haven’t researched well up to now.

Each Embassy has their own way of doing things, so search embassy specific.