Can I do a border run after obtaining a 6-month tourist visa from the Thai embassy in the U.S.?

Hi guys I'm from America and got a 6 month tourist visa from the thai embassy located in the United States of America before arriving in Thailand.

I get a 60 day stamp when i arrive at the airport.

I'm supposed to get it 3 times which makes it 6 months.

Now i have to leave the county on October 8th.

Does anyone know if i can do a border run again?

i did the border run twice when i first arrived in Thailand, in mai sai.

I know you can't do border runs more than twice per year, but does that only apply only if you don't set up a visa before arriving in Thailand?

Or even if you got a 6 month visa before arriving in Thailand from the Thai us embassy located in the United States of America like i did, would i still be prohibited from doing a border run? Even if i went somewhere else besides mai sae?

Please let me know i am anxious about this, i looked online but can't seem to find any solid answer for my specific case.

Thank you all who help.