Do I need to have the entire 500,000 baht in one bank account for my visa application?

The funds do not need to be in a single bank account for your visa application. You can present evidence of the 500,000 baht across multiple accounts, as long as all accounts are in your name and accessible. Ensure that the records are clear and well-organized to facilitate understanding by the Embassy staff. It's advisable that the funds be in personal savings or liquid accounts and that three months of bank statements for all accounts are provided.